Role of cyclopentenone prostaglandins in rat carrageenin pleurisy

FEBS Letters

Di Rosa, Massimo¹  Ialenti, Armando¹  Ianaro, Angela¹  Maffia, Pasquale¹  Pisano, Barbara¹ 

[1] Department of Experimental Pharmacology, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy

关键词: Carrageenin pleurisy;    Cyclooxygenase inhibitor;    Heat shock factor;    Heat shock protein;    Nuclear factor-κB;    Transcription factor;    COX;    cyclooxygenase;    cyPGs;    cyclopentenone prostaglandins;    EMSA;    electrophoretic mobility shift assay;    HSF1;    heat shock factor 1;    hsp72;    heat shock protein 72;    NSAIDs;    non-steroidal anti-inflammatory drugs;    NF-κB;    nuclear factor κB;    PPAR-γ;    peroxisome proliferator-activated receptor-γ;    PGs;    prostaglandins;   

DOI  :  10.1016/S0014-5793(01)03035-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, using rat carrageenin-induced pleurisy, we found that treatment of rats with either indomethacin or NS-398 suppressed the pleurisy at 2 h but significantly exacerbated this reaction at 48 h. Exacerbated inflammation was associated with reduced prostaglandin D₂ levels, decreased heat shock factor 1 (HSF1) activation, reduced hsp72 expression and increased activation of nuclear factor κB (NF-κB). Replacement of cyclopentenone prostaglandins by treating rats with either prostaglandin J₂ or prostaglandin D₂ reversed the exacerbating effects of cyclooxygenase inhibitors leading to the resolution of the reaction. In conclusion, we demonstrate that cyclopentenone prostaglandins may act as anti-inflammatory mediators by inducing in inflammatory cells HSF1-dependent hsp72 expression and NF-κB inhibition, two crucial events for the remission of inflammation.

【 授权许可】

Unknown