FEBS Letters | |
Inhibition of isoprenoid biosynthesis causes insulin resistance in 3T3‐L1 adipocytes | |
Chamberlain, Luke H1  | |
[1] Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK | |
关键词: Glut4; Glut1; Lovastatin; Cholesterol; Isoprenoid; Adipocyte; HMG-CoA; 3-hydroxy-3-methylglutaryl CoA; Glut; glucose transporter; IRAP; insulin-responsive aminopeptidase; | |
DOI : 10.1016/S0014-5793(01)03007-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Lovastatin treatment caused down-regulation of the insulin-responsive glucose transporter 4 (Glut4) and up-regulation of Glut1 in 3T3-L1 adipocytes. These changes in protein expression were associated with a marked inhibition of insulin-stimulated glucose transport. Lovastatin had no effect on cell cholesterol levels, but its effects were reversed by mevalonate, demonstrating that inhibition of isoprenoid biosynthesis causes insulin resistance in 3T3-L1 adipocytes. These findings support the notion that whole body insulin resistance may arise as a result of perturbations in general biochemical pathways, rather than primary defects in insulin signalling.
【 授权许可】
Unknown
【 预 览 】
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