Inhibition of isoprenoid biosynthesis causes insulin resistance in 3T3‐L1 adipocytes

FEBS Letters

Chamberlain, Luke H¹ 

[1] Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK

关键词: Glut4;    Glut1;    Lovastatin;    Cholesterol;    Isoprenoid;    Adipocyte;    HMG-CoA;    3-hydroxy-3-methylglutaryl CoA;    Glut;    glucose transporter;    IRAP;    insulin-responsive aminopeptidase;   

DOI  :  10.1016/S0014-5793(01)03007-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lovastatin treatment caused down-regulation of the insulin-responsive glucose transporter 4 (Glut4) and up-regulation of Glut1 in 3T3-L1 adipocytes. These changes in protein expression were associated with a marked inhibition of insulin-stimulated glucose transport. Lovastatin had no effect on cell cholesterol levels, but its effects were reversed by mevalonate, demonstrating that inhibition of isoprenoid biosynthesis causes insulin resistance in 3T3-L1 adipocytes. These findings support the notion that whole body insulin resistance may arise as a result of perturbations in general biochemical pathways, rather than primary defects in insulin signalling.

【 授权许可】

Unknown