Role of Flk‐1 in mouse hematopoietic stem cells

FEBS Letters

Todokoro, Kazuo¹  Nagata, Yuka¹  Haruta, Hirotaka¹ 

[1] Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

关键词: KDR;    Flk-1;    Stem cell;    CD34;    SP;    side population;    PI;    propidium iodide;    PBS;    phosphate-buffered saline;    FCS;    fetal calf serum;   

DOI  :  10.1016/S0014-5793(01)02921-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It was reported that human hematopoietic stem cells in bone marrow were restricted to the CD34⁺KDR⁺ cell fraction. We found that expression levels of Flk-1, a mouse homologue of KDR, were low or undetectable in mouse Lin⁻c-Kit⁺Sca-1⁺CD34^low/− cells as well as Hoechst33342⁻ cells (side population), which have long-term reconstitution capacity. Furthermore, neither Flk-1⁺CD34^low/− cells nor Flk-1⁺CD34⁺ cells had long-term reconstitution capacity in mouse. Taken together with other observations using Flk-1-deficient mice, these results indicate that Flk-1 is essential for the development of hematopoietic stem cells in embryo but not for the function of hematopoietic stem cells in adult mouse bone marrow.

【 授权许可】

Unknown