Signalling of GPI‐anchored CD157 via focal adhesion kinase in MCA102 fibroblasts

FEBS Letters

Chang, Chan Fong¹  Liang, Fubo¹ 

[1] Department of Biochemistry, Faculty of medicine, National University of Singapore, Singapore 119260

关键词: CD157;    Focal adhesion kinase;    Localization;    Morphology;    Proliferation;    Tyrosine phosphorylation;    GPI;    glycosylphosphatidylinositol;    FAK;    focal adhesion kinase;    cADPR;    cyclic ADP ribose;    uPAR;    urokinase-type plasminogen activator receptor;   

DOI  :  10.1016/S0014-5793(01)02912-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD157, a glycosylphosphatidylinositol-anchored protein, has previously been shown to mediate tyrosine phosphorylation of a 130 kDa protein (p130) in several cell lines. In this study, we have identified the p130 protein to be focal adhesion kinase (FAK or pp125^FAK). FAK undergoes phosphorylation at Tyr-397 and Tyr-861 in intact MCA102 cells stably transfected with CD157 (MCA/CD157). MCA/CD157 cells, which displayed a rounded and compact cell morphology, exhibited a dispersed distribution, in contrast to a more closely associated and elongated spindle cell shape in the vector-transfected cells. MCA/CD157 cells proliferated at a rate 20–25% slower than the control cells. Our results demonstrate, for the first time, that FAK is a downstream signalling molecule of CD157.

【 授权许可】

Unknown