CD157 in bone marrow mesenchymal stem cells mediates mitochondrial production and transfer to improve neuronal apoptosis and functional recovery after spinal cord injury

Stem Cell Research & Therapy

Jing Li¹  Simin Cai¹  Heyangzi Li¹  Xiaoming Zhang²  Shi Bai³  Huabo Cai⁴ 

[1] Department of Anatomy, School of Medicine, Zhejiang University, 310058, Hangzhou, China;Department of Anatomy, School of Medicine, Zhejiang University, 310058, Hangzhou, China;Department of Emergency Medicine, Sir Run Run Shaw Hospital; School of Medicine, Zhejiang University, 310058, Hangzhou, China;Department of Anatomy, Taizhou University; School of Medicine, Zhejiang University, 310058, Hangzhou, China;Department of Emergency Medicine, Sir Run Run Shaw Hospital; School of Medicine, Zhejiang University, 310058, Hangzhou, China;

关键词: Spinal cord injury;    Bone marrow stromal cells;    Mitochondria;    CD157;    Calcium;   

DOI  :  10.1186/s13287-021-02305-w

来源: Springer

【 摘 要 】

BackgroundRecent studies demonstrated that autologous mitochondria derived from bone marrow mesenchymal stem cells (BMSCs) might be valuable in the treatment of spinal cord injury (SCI). However, the mechanisms of mitochondrial transfer from BMSCs to injured neurons are not fully understood.MethodsWe modified BMSCs by CD157, a cell surface molecule as a potential regulator mitochondria transfer, then transplanted to SCI rats and co-cultured with OGD injured VSC4.1 motor neuron. We detected extracellular mitochondrial particles derived from BMSCs by transmission electron microscope and measured the CD157/cyclic ADP-ribose signaling pathway-related protein expression by immunohistochemistry and Western blotting assay. The CD157 ADPR-cyclase activity and Fluo-4 AM was used to detect the Ca2+ signal. All data were expressed as mean ± SEM. Statistical analysis was analyzed by GraphPad Prism 6 software. Unpaired t-test was used for the analysis of two groups. Multiple comparisons were evaluated by one-way ANOVA or two-way ANOVA.ResultsCD157 on BMSCs was upregulated when co-cultured with injured VSC4.1 motor neurons. Upregulation of CD157 on BMSCs could raise the transfer extracellular mitochondria particles to VSC4.1 motor neurons, gradually regenerate the axon of VSC4.1 motor neuron and reduce the cell apoptosis. Transplantation of CD157-modified BMSCs at the injured sites could significantly improve the functional recovery, axon regeneration, and neuron apoptosis in SCI rats. The level of Ca2+ in CD157-modified BMSCs dramatically increased when objected to high concentration cADPR, ATP content, and MMP of BMSCs also increased.ConclusionThe present results suggested that CD157 can regulate the production and transfer of BMSC-derived extracellular mitochondrial particles, enriching the mechanism of the extracellular mitochondrial transfer in BMSCs transplantation and providing a novel strategy to improve the stem cell treatment on SCI.

【 授权许可】

CC BY