Mimicking phosphorylation at Ser‐48 strongly reduces surface expression of human macrophage scavenger receptor class A: implications on cell motility

FEBS Letters

Heider, Harald¹  Wintergerst, Eva S¹ 

[1]Institute of Biochemistry, University of Basel, Vesalgasse 1, 4051 Basel, Switzerland

关键词: Scavenger receptor;    Macrophage;    Acetylated low density lipoprotein;    Cell motility;    Surface expression;    SRA1;    macrophage scavenger receptor A1;    EGFP;    enhanced green fluorescent protein;    LDL;    low density lipoprotein;    AcLDL;    acetylated LDL;    TR-AcLDL;    Texas red-labeled acetylated LDL;    OxLDL;    oxidized LDL;    PBS;    phosphate-buffered saline;    PBST;    PBS supplemented with 0.1% Tween 20;   

DOI  :  10.1016/S0014-5793(01)02819-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of human macrophage scavenger receptor A1 (SRA1) in the development of atherosclerotic lesions is still scarcely defined. Substituting serine 48 in human SRA1 by an aspartate demonstrated that (1) surface expression of the mutated receptor was 13-fold decreased; (2) the amount of cell-associated Texas red-labeled acetylated low density lipoprotein (LDL) in mutant receptor-expressing cells was almost three-fold reduced; (3) the migration of mutant receptor-transfected cells towards surfaces coated with oxidized LDL decreased by almost 60% compared to cells that were transfected with the wild type receptor. Phosphorylation of the cytoplasmic part of SRA1 may help to modulate the residence time of macrophages in atherosclerotic lesions.

【 授权许可】

Unknown