Internalization‐defective mutants of somatostatin receptor subtype 2 exert normal signaling functions in hematopoietic cells

FEBS Letters

Oomen, Sigrid P.M.A.²  Hofland, Leo J.¹  Lamberts, Steven W.J.¹  Löwenberg, Bob²  Touw, Ivo P.² 

[1] Department of Internal Medicine, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands;Institute of Hematology, Erasmus University Rotterdam (Room H Ee 1314), P.O. Box 1738, 3000 DR Rotterdam, The Netherlands

关键词: Somatostatin;    Receptor subtype;    Internalization;    Hematopoietic cell;    Chemoattractant;    GPCR;    G-protein-coupled receptor;    GRK;    G-protein receptor kinase;    PKA;    protein kinase A;    PKC;    protein kinase C;    SST;    somatostatin;    SSTR;    somatostatin receptor;    wt;    wild-type;   

DOI  :  10.1016/S0014-5793(01)02729-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The regulatory peptide somatostatin (SST) acts via a family of G-protein-coupled receptors comprising five subtypes (SSTR1–5). G-protein-coupled receptors activate multiple signaling mechanisms, which variably depend on internalization and intracellular routing of activated receptors. We have recently demonstrated that hematopoietic precursors express SSTR2 and that SST is a chemoattractant for these cells. Herein, we characterize critical regions in SSTR2 involved in endocytosis and describe how ligand-induced internalization impacts on two major signaling functions of SSTR2 in hematopoietic cells, the activation of the Erk pathway and the induction of promigratory responses.

【 授权许可】

Unknown