β‐Arrestin is involved in the desensitization but not in the internalization of the somatostatin receptor 2A expressed in CHO cells

FEBS Letters

Vincent, Jean-Pierre¹  Guillen, Stéphanie¹  Mazella, Jean¹  Brasselet, Sabrina¹ 

[1] Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, UMR 6097, 660 route des Lucioles, 06560 Valbonne, France

关键词: Somatostatin;    Receptor;    β-Arrestin;    Internalization;    Desensitization;    SRIF;    somatotropin release inhibitory factor;    sst2A;    SRIF receptor type 2A;    GPCR;    G protein-coupled receptor;    PheAsO;    phenyl arsine oxide;   

DOI  :  10.1016/S0014-5793(02)02517-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of β-arrestin-1 with the somatostatin receptor type 2A (sst2A) was monitored using both biochemical and confocal imaging approaches. We show that, using transient transfection of either β-arrestin-1 or its dominant negative Δ-arrestin-1 in CHO cells stably transfected with the sst2A, β-arrestin-1 is colocalized with the receptor in endosomal vesicles after somatostatin-induced sequestration. However, this interaction leads to a role of β-arrestin-1 in the desensitization of the sst2A rather than in the internalization process of the receptor–ligand complex.

【 授权许可】

Unknown