Autocatalytic activation of human legumain at aspartic acid residues

FEBS Letters

Halfon, Sherin¹  Vega, Felix¹  Zurawski, Gerard¹  Patel, Sejal¹  Zurawski, Sandra¹ 

[1] DNAX Research Institute, 901 California Avenue, Palo Alto, CA 94304-1104, USA

关键词: Cysteine protease;    Legumain;    Hematopoietic cell;   

DOI  :  10.1016/S0014-5793(98)01281-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human legumain was characterized following overexpression in a murine cell line as the C-terminal Ig-fusion protein. Upon acid treatment, the prolegumain autoproteolyzed distal to two aspartic acid residues to yield a highly active form. The ability of mature legumain to cleave after aspartic acid residues was confirmed with a small peptide substrate. Substitution of alanine for the putative catalytic cysteine, or for either of two strictly conserved histidine residues, partly or wholly eliminated autoactivation but not the ability of wild-type legumain to correctly process the variants to the properly sized proteins.

【 授权许可】

Unknown