Rab3a controls exocytosis in cholecystokinin‐secreting cells

FEBS Letters

Gevrey, Jean-Claude¹  Chayvialle, Jean-Alain¹  Regazzi, Romano²  Abello, Jacques¹  Laurent, Séverine¹  Chevrier, Anne-Marie¹  Saurin, Jean-Christophe¹  Némoz-Gaillard, Eric¹ 

[1] INSERM Unité 45, Hôpital Edouard Herriot, Pavillon H, F-69437 Lyon Cedex 3, France;Institut de Biologie Cellulaire et de Morphologie, Rue du Bugnon 9, CH-1005 Lausanne, Switzerland

关键词: Exocytosis;    rab3;    Cholecystokinin;    Enteroendocrine cell;    CCK;    cholecystokinin;    hGH;    human growth hormone;    SNARE;    soluble N-ethylmaleimide-sensitive factor attachment protein receptor;   

DOI  :  10.1016/S0014-5793(01)02683-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of rab3A and rab3D isoforms in the enteroendocrine, cholecystokinin-secreting, cell lines STC-1 and GLUTag is here demonstrated. In contrast, rab3B is undetectable in these two cell lines, and rab3C is only slightly expressed in GLUTag cells. Using a transient co-transfection system with human growth hormone as reporter protein, we show that overexpression of the GTPase-deficient mutant rab3AQ81L, but not rab3DQ81L, significantly decreases human growth hormone secretory responses to various agonists in STC-1 cells. These results indicate that endocrine cell lines of intestinal origin express rab3A and rab3D proteins, but the GTP-bound form of rab3A only acts as a negative modulator in the control of cholecystokinin secretion from STC-1 cells.

【 授权许可】

Unknown