FEBS Letters | |
Expression of SNARE proteins in enteroendocrine cell lines and functional role of tetanus toxin‐sensitive proteins in cholecystokinin release | |
Takahashi, Masami2  Chayvialle, Jean-Alain1  Regazzi, Romano3  Cuber, Jean-Claude1  Catsicas, Stefan3  Abello, Jacques1  Bernard, Christine1  Bosshard, Arlette1  Némoz-Gaillard, Eric1  | |
[1] INSERM Unité 45, Hôpital Edouard-Herriot, Pavillon Hbis, 69437 Lyon Cédex 3, France;Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan;Institut de Biologie Cellulaire et de Morphologie, Rue du Bugnon 9, 1005 Lausanne, Switzerland | |
关键词: Syntaxin; Synaptosomal-associated protein of 25 kDa; Vesicle-associated membrane protein; Tetanus toxin; Enteroendocrine cell; Cholecystokinin exocytosis; CCK; cholecystokinin; SNAP-25; synaptosomal-associated protein of 25 kDa; VAMP; vesicle-associated membrane protein; SL-O; streptolysin-O; Tetx; tetanus toxin; | |
DOI : 10.1016/S0014-5793(98)00209-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In neurons, synaptic vesicle exocytosis involves the formation of a core complex particle including syntaxin-1, synaptosomal-associated protein of 25 kDa (SNAP-25) and vesicle-associated membrane protein (VAMP)-2/synaptobrevin. The expression of these proteins was investigated in a panel of cell lines, including lines of endocrine and intestinal origin, by Western blotting and/or immunocytochemistry. The three core complex proteins were detected in the enteroendocrine, cholecystokinin (CCK)-secreting, cell lines STC-1 and GLUTag, and in the endocrine non-intestinal cell lines CA-77 and HIT-T15. In contrast, SNAP-25 and syntaxin-1 were undetected in the intestinal non-endocrine cell lines IEC-6, HT-29 and Caco-2, whereas a slight expression of VAMP-2 was documented in IEC-6 and HT-29 cells. Co-immunoprecipitation experiments indicated that syntaxin-1, SNAP-25 and VAMP-2 were present in a complex similar to that identified in brain. In the STC-1 cell line, treatment of streptolysin-O-permeabilized cells with tetanus toxin (Tetx) selectively cleaved VAMP-2 and VAMP-3/cellubrevin, and simultaneously abolished Ca2+-induced CCK secretion (IC50∼12 nM). These results show that endocrine cell lines of intestinal origin express syntaxin-1, SNAP-25 and VAMP-2, and suggest a key role for a Tetx-sensitive protein (for example VAMP-2 and/or VAMP-3) in the CCK secretion by STC-1 cells.
【 授权许可】
Unknown
【 预 览 】
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