rab3‐Peptide stimulates exocytosis from mast cells via a pertussis toxin‐sensitive mechanism

FEBS Letters

Law, G.J.¹  Northrop, A.J.¹  Mason, W.T.¹ 

[1] Laboratory of Neural and Secretory Signalling, Department of Neurobiology, AFRC, Babraham Institute, Babraham, Cambridge CB2 4 AT, UK

关键词: Exocytosis;    rab3;    GTP-binding protein;    Pertussis toxin;    Mast cell;   

DOI  :  10.1016/0014-5793(93)80374-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic peptides of the putative effector domain of members of the rab3 gene family of small GTP-binding proteins have been shown to have potent actions on vesicular transport and exocytosis [1,2]. Here, we use similar rab3-effector domain peptides to study their role in intracellular signalling in mast cells. We find that rab3-like peptides stimulate exocytosis and decrease cyclic 3',5'-AMP levels in these cells when applied extracellularly. Cells pretreated with pertussis toxin (PtX) to selectively uncouple α_i/α₀ type G proteins from their biological activators, however, did not respond to rab3 peptides. rab3-like peptides also induce a Ca²⁺ transient in mast cells. These observations provide evidence for functional coupling between an effector domain peptide sequence of rab3 protein and a PtX-sensitive G protein substrate.

【 授权许可】

Unknown