Transcriptional effect of hypoxia on placental leptin

FEBS Letters

André, Jocelyne²  Grosfeld, Alexandra²  Cauzac, Michèle³  Hauguel-de Mouzon, Sylvie³  Challier, Jean-Claude¹  Turban, Sophie²  Guerre-Millo, Michèle² 

[1] Université Pierre et Marie Curie, Upres EA2396, 4 Place Jussieu, Paris, France;INSERM U 465, 15 rue de l'Ecole de Médecine, 75006 Paris, France;CNRS UPR 1524, 24 rue du Faubourg St Jacques, Paris, France

关键词: Placenta;    Leptin;    Preeclampsia;    Hypoxia;    BeWo cell;    Hypoxia-inducible factor-1;   

DOI  :  10.1016/S0014-5793(01)02673-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We observed recently1 that placental leptin is markedly increased in preeclampsia. Since this disorder is associated with vascular disorders, we have tested the hypothesis that hypoxia regulates leptin expression. We show that hypoxia increased leptin mRNA and secretion in trophoblast-derived BeWo cells. This effect was mediated through leptin promoter activation. 5′ deletion analysis allowed us to delineate two regions containing 1.87 kb and 1.20 kb of the promoter which conferred respectively high and low responsiveness to hypoxia. These data indicate that leptin is up-regulated by hypoxia through a transcriptional mechanism likely to involve distinct hypoxia-responsive cis-acting sequences on the promoter.

【 授权许可】

Unknown