Visualizing detergent resistant domains in model membranes with atomic force microscopy

FEBS Letters

Rinia, Hilde A.²  de Kruijff, Ben²  Snel, Margot M.E.¹  van der Eerden, Jan P.J.M.¹ 

[1] Department of Interfaces, Debye Institute, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands;Department of Biochemistry of Membranes, CBLE, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: Raft;    Sphingolipid;    Cholesterol;    Phase separation;    Planar lipid bilayer;    Percolating;    DRM;    detergent resistant membrane;    AFM;    atomic force microscopy;    SpM;    sphingomyelin;    T m;    melting temperature;    DOPC;    1;    2-dioleoyl-sn-glycero-3-phosphocholine;    SUV;    small unilamellar vesicle;    DSC;    differential scanning calorimetry;    TLC;    thin layer chromatography;   

DOI  :  10.1016/S0014-5793(01)02636-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Evidence is accumulating that in cell membranes microdomains exist, also referred to as rafts or detergent resistant membranes. In this study, atomic force microscopy is used to study supported lipid bilayers, consisting of a fluid phosphatidylcholine, sphingomyelin and cholesterol. Domains were visualized of which the morphology and size depended on the cholesterol concentration. The presence of cholesterol was found to induce bilayer coupling. At 30 mol% cholesterol, a change in percolation phase was observed, and at 50 mol%, when both fluid lipids and solid lipids are saturated with cholesterol, phase separation was still observed. In addition, we were able to directly visualize the resistance of domains against non-ionic detergent.

【 授权许可】

Unknown