Sphingomyelin is much more effective than saturated phosphatidylcholine in excluding unsaturated phosphatidylcholine from domains formed with cholesterol

FEBS Letters

Chupin, Vladimir¹  van Duyl, Bianca Y.¹  Killian, J.Antoinette¹  de Kruijff, Ben¹  Ganchev, Dragomir¹ 

[1] Department of Biochemistry of Membranes, CBLE, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: 2H nuclear magnetic resonance;    Atomic force microscopy;    Raft;    Model membrane;    Sphingolipid;    Glycerophospholipid;    DOPC;    1;    2-dioleoyl-sn-glycero-3-phosphocholine;    DPPC;    1;    2-dipalmitoyl-sn-glycero-3-phosphocholine;    SM;    sphingomyelin;    NMR;    nuclear magnetic resonance;    AFM;    atomic force microscopy;    Lo;    liquid-ordered;    Ld;    liquid-disordered;    T m;    transition temperature;   

DOI  :  10.1016/S0014-5793(03)00678-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, we compared domain formation in raft-like mixtures of cholesterol and dioleoylphosphatidylcholine (DOPC) with either sphingomyelin (SM) or dipalmitoylphosphatidylcholine (DPPC). Using ²H nuclear magnetic resonance, we studied the properties of the lipid enriched in the fluid phase, DOPC. We found that acyl chain ²H-labeled DOPC is much less ordered in SM-containing mixtures than in those containing DPPC, suggesting that DOPC in the SM-containing mixture senses a lower concentration of cholesterol in its direct environment. Atomic force microscopy experiments demonstrated large differences in the size and shape of domains in the different mixtures. We propose that these various differences are a consequence of the preferential interaction of cholesterol for sphingolipids over glycerophospholipids.

【 授权许可】

Unknown