Expression of membrane C1q in human monocyte‐derived macrophages is developmentally regulated and enhanced by interferon‐γ

FEBS Letters

Loos, Michael¹  Kaul, Marcus¹ 

[1] Institute of Medical Microbiology and Hygiene, Johannes-Gutenberg University, Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany

关键词: Monocyte/macrophage;    Differentiation;    Complement;    C1q;    Phagocytosis;    ABS;    human AB serum;    mC1q;    membrane C1q;    MFI;    mean fluorescence intensity;    rMFI;    relative MFI;    RT-PCR;    reverse transcriptase-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(01)02592-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study investigated when during ‘in vitro’ maturation macrophages (MΦ) express membrane C1q (mC1q), and whether cell activation affects expression and function of mC1q. Although C1q mRNA was repeatedly detected in freshly isolated monocytes using reverse transcriptase-polymerase chain reaction, C1q protein was observed only in developing MΦ from day 1 to 4 on using immunodetection and flow cytometry. However, the quantity of mC1q and other MΦ membrane proteins differed strikingly in cells from different donors. We report here for the first time that CD14⁺ and CD14⁻ mC1q-bearing MΦ can develop, and that interferon-γ increases mC1q display at the cell surface, and mC1q-mediated phagocytosis.

【 授权许可】

Unknown