Fibronectin is a binding partner for the myelin‐associated glycoprotein (siglec‐4a)

FEBS Letters

Kelm, Sørge²  Schauer, Roland³  Brossmer, Reinhard¹  Ihrig, Petra¹  Strenge, Karen³ 

[1]Biochemistry Centre Heidelberg (BZH), University of Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany

[2]Department of Biology and Chemistry, University Bremen, P.O. Box 330440, D-28334 Bremen, Germany

[3]Institute of Biochemistry, University of Kiel, Olshausenstrasse 40, D-24098 Kiel, Germany

关键词: Myelin-associated glycoprotein;    Siglec;    Sialic acid;    Cellular interaction;    Extracellular matrix;    Fibronectin;    ECM;    extracellular matrix;    FCS;    fetal calf serum;    Fn;    fibronectin;    MAG;    myelin-associated glycoprotein;    MAG-Fc;    recombinant extracellular domains of MAG fused to the Fc fragment of human immunoglobulin;   

DOI  :  10.1016/S0014-5793(01)02566-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The myelin-associated glycoprotein (MAG) mediates cell–cell interactions between myelinating glial cells and neurons. Here we describe the extracellular matrix glycoprotein fibronectin as a binding partner of MAG. It has been identified by affinity precipitation with MAG-Fc from NG108-15 cells and by microsequencing of two peptides derived from a 210-kDa protein band. Western blot analysis showed that fibronectin is also present in MAG binding partners isolated from N₂A (murine neuroblastoma) cells, rat brain and rat spinal cord. Different fibronectin isoforms have been isolated from brains of young and adult rats, indicating that the expression of MAG binding fibronectin changes during development.

【 授权许可】

Unknown