A novel plant α4‐fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewisa adhesion determinant

FEBS Letters

Costa, Júlia¹  Vila-Verde, Cidália¹  Palma, Angelina S.¹  Pires, Ana Sofia¹  Fevereiro, Pedro S.¹ 

[1] Instituto de Tecnologia Quı́mica e Biológica, Apartado 127, 2781-901 Oeiras, Portugal

关键词: Plant complex glycan;    Type I motif;    Lewisa;    Fucosyltransferase;    Vaccinium myrtillus;    Fuc;    fucose;    Gal;    galactose;    GlcNAc;    N-acetylglucosamine;    Lea;    Lewisa;    NEM;    N-ethylmaleimide;    α4-FucT;    α4-fucosyltransferase;   

DOI  :  10.1016/S0014-5793(01)02568-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have partially characterised an α4-fucosyltransferase (α4-FucT) from Vaccinium myrtillus, which catalysed the biosynthesis of the Lewis^a adhesion determinant. The enzyme was stable up to 50°C. The optimum pH was 7.0, both in the presence and in the absence of Mn²⁺. The enzyme was inhibited by Mn²⁺ and Co²⁺, and showed resistance towards inhibition with N-ethylmaleimide. It transferred fucose to N-acetylglucosamine in the type I Galβ3GlcNAc motif from oligosaccharides linked to a hydrophobic tail and glycoproteins (containing the type I motif). Sialylated oligosaccharides containing the type II Galβ4GlcNAc motif were not acceptors. The catalytic mechanism of the plant α4-FucT possibly involves a His residue, and it must have arisen by convergent evolution relative to its mammalian counterparts.

【 授权许可】

Unknown