Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)

FEBS Letters

Melo, Nuno S¹  Conradt, Harald S²  Fevereiro, Pedro S¹  Costa, Júlia¹  Nimtz, Manfred² 

[1] ITQB/IBET, Apartado 12, P-2780 Oeiras, Portugal;GBF, Mascheroder Weg 1, D-38124 Braunschweig, Germany

关键词: Extracellular glycoprotein;    Lewisa;    Peroxidase;    Plant complex oligosaccharide;    Vaccinium myrtillus;    ESI-MS;    electrospray ionization mass spectrometry;    Fuc;    fucose;    Gal;    galactose;    GlcNAc;    N-acetylglucosamine;    Lea;    Lewisa;    Lex;    Lewisx;    MALDI/TOF-MS;    matrix assisted laser desorption/ionization time of flight mass spectrometry;    Man;    mannose;    MS;    mass spectrometry;    PNGase A;    peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidase A;    VMPxC1 or C1;    Vaccinium myrtillus peroxidase C1;    Xyl;    xylose;   

DOI  :  10.1016/S0014-5793(97)01121-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This paper reports for the first time the presence of the human Lewis^a type determinant in glycoproteins secreted by plant cells. A single glycopeptide was identified in the tryptic hydrolysis of the peroxidase VMPxC1 from Vaccinium myrtillus L. by HPLC/ESI-MS. The oligosaccharide structures were elucidated by ESI-MS-MS and by methylation analysis before and after removal of fucose by mild acid hydrolysis. The major structure determined is of the biantennary plant complex type containing the outer chain motif Lewis^a

【 授权许可】

Unknown