Interference of a commercial catalase preparation in laccase and peroxidase activities

Brazilian Archives of Biology and Technology

Nara Ballaminut²  Renata Yamanaka¹  Katia Maria Gomes Machado¹ 

[1] ,Instituto de Botânica Secretaria do Meio Ambiente do Estado São Paulo SP ,Brasil

关键词: Peroxidase;    fungi;    basidiomycetes;    hydrogen peroxide;    enzymatic inhibition;   

DOI  :  10.1590/S1516-89132009000500017

来源: SciELO

【 摘 要 】

The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.

【 授权许可】

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