Overexpression of Bcl‐2 suppresses the calcium activation of a mitochondrial megachannel

FEBS Letters

Schneider, Erasmus²  Kinnally, Kathleen W.¹  Murphy, Robert C.² 

[1] Department of Basic Sciences, New York University College of Dentistry, 345 E. 24th St., New York, NY 10010, USA;Molecular Medicine, Wadsworth Center, Empire State Plaza, P.O. Box 509, Albany, NY 12201-0509, USA

关键词: Mitochondrion;    Apoptosis;    Bcl-2;    Patch clamp;    Channel;    MCC;    multiple conductance channel;   

DOI  :  10.1016/S0014-5793(01)02440-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular mechanism(s) by which Bcl-2 regulates apoptosis is poorly understood. Bcl-2 suppresses apoptosis by inhibiting calcium activation of the permeability transition of mitochondria. In this patch-clamp study, overexpression of Bcl-2 in mitochondria of cultured cells suppressed calcium activation of a high conductance channel that may underlie the permeability transition. All other single channel parameters were identical when multiple conductance channel activities of mitochondria from control and Bcl-2 overexpressing cells were compared. Bcl-2 forms channels in artificial membranes; however, no novel channel activities could be linked to Bcl-2 overexpression, suggesting Bcl-2 does not form channels in native inner membranes of mitochondria.

【 授权许可】

Unknown