FEBS Letters | |
Overexpression of Bcl‐2 suppresses the calcium activation of a mitochondrial megachannel | |
Schneider, Erasmus2  Kinnally, Kathleen W.1  Murphy, Robert C.2  | |
[1] Department of Basic Sciences, New York University College of Dentistry, 345 E. 24th St., New York, NY 10010, USA;Molecular Medicine, Wadsworth Center, Empire State Plaza, P.O. Box 509, Albany, NY 12201-0509, USA | |
关键词: Mitochondrion; Apoptosis; Bcl-2; Patch clamp; Channel; MCC; multiple conductance channel; | |
DOI : 10.1016/S0014-5793(01)02440-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The molecular mechanism(s) by which Bcl-2 regulates apoptosis is poorly understood. Bcl-2 suppresses apoptosis by inhibiting calcium activation of the permeability transition of mitochondria. In this patch-clamp study, overexpression of Bcl-2 in mitochondria of cultured cells suppressed calcium activation of a high conductance channel that may underlie the permeability transition. All other single channel parameters were identical when multiple conductance channel activities of mitochondria from control and Bcl-2 overexpressing cells were compared. Bcl-2 forms channels in artificial membranes; however, no novel channel activities could be linked to Bcl-2 overexpression, suggesting Bcl-2 does not form channels in native inner membranes of mitochondria.
【 授权许可】
Unknown
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