FEBS Letters | |
Evolution and function of the neisserial dam‐replacing gene | |
Bruni, Carmelo Bruno1  Lavitola, Alfredo1  Pagliarulo, Caterina1  Alifano, Pietro2  Salvatore, Paola1  Bucci, Cecilia1  Roberti, Vera1  Cantalupo, Giuseppina1  | |
[1] Dipartimento di Biologia e Patologia Cellulare e Molecolare ‘L. Califano’, Università di Napoli ‘Federico II’, and Centro di Endocrinologia ed Oncologia Sperimentale ‘G. Salvatore’, Consiglio Nazionale delle Ricerche, Via S. Pansini 5, 80131 Naples, Italy;Dipartimento di Biologia, Università degli Studi di Lecce, Via Monteroni, 73100 Lecce, Italy | |
关键词: Type II restriction endonuclease; NmeBII; DNA repair; Neisserial small repeated element; Non-pathogenic Neisseria; Neisseria meningitidis; Neisseria lactamica; | |
DOI : 10.1016/S0014-5793(01)02388-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Phase variation through slippage-like mechanisms involving homopolymeric tracts depends in part on the absence of Dam-methylase in several pathogenic isolates of Neisseria meningitidis. In Dam-defective strains drg (dam-replacing gene), flanked by pseudo-transposable small repeated elements (SREs), replaced dam. We demonstrate that drg encodes a restriction endonuclease (NmeBII) that cleaves 5′-GmeATC-3′. drg is also present in 50% of Neisseria lactamica strains, but in most of them it is inactive because of the absence of an SRE-providing promoter. This is associated with the presence of GATmeC, suggesting an alternative restriction-modification system (RM) specific for 5′-GATC-3′, similar to Sau3AI-RM of Staphylococcus aureus 3A, Lactococcus lactis KR2 and Listeria monocytogenes.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020310519ZK.pdf | 317KB | download |