Functional role of the HIV‐1 Rev exon 1 encoded region in complex formation and trans‐dominant inhibition

FEBS Letters

Gjerdrum, Christine¹  Stranda, Annicke¹  Szilvay, Anne Marie¹ 

[1] Department of Molecular Biology, University of Bergen, HIB, P.O. Box 7800, N-5020 Bergen, Norway

关键词: Human immunodeficiency virus type 1;    Rev;    Tev;    Oligomerization;    Trans-dominant inhibition;    HIV-1;    human immunodeficiency virus type 1;    Env;    HIV-1 glycoproteins;    LTR;    long terminal repeat;    NES;    nuclear export signal;    MAb;    monoclonal antibody;    CHO;    Chinese hamster ovary cells;    COS;    African green monkey kidney cell line transformed by SV40;   

DOI  :  10.1016/S0014-5793(01)02364-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study functional aspects of the exon 1 encoded region of the human immunodeficiency virus type 1 Rev protein, the viral Tev protein which exhibits low Rev activity but lacks the rev exon 1 encoded region was examined. Neither Rev–Tev heteromer complex formation nor inhibition of Rev by an export deficient Tev mutant was observed. Insertion of the rev exon 1 encoded region into the Tev mutant allowed it to oligomerize with Rev and act as a trans-dominant negative mutant. This showed that the exon 1 encoded region of Rev is essential for oligomerization and that oligomerization is a prerequisite for trans-dominant inhibition.

【 授权许可】

Unknown