Biochemical characterization of HIV‐1 Rev as a potent activator of casein kinase II in vitro

FEBS Letters

Harada, Shigeyoshi²  Ito, Masahiko¹  Ohtsuki, Kenzo²  Karino, Atsushi²  Morikawa, Yuko³  Maekawa, Toshiro² 

[1]Department of Microbiology, Yamanashi Medical University, Shimokatoh 1110, Tamaho-chyo, Yamanashi 409-3821, Japan

[2]Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences, Kitasato 1-15-1, Sagamihara 228-8555, Japan

[3]The Kitasato Institute, Shirokane 5-9-1, Tokyo 108-8641, Japan

关键词: Casein kinase II;    Casein kinase-II activator;    Casein kinase-II inhibitor;    Human immunodeficiency virus-1;    Human immunodeficiency virus-1 regulator of expression of virion proteins;    Human MOLT-4 cell;    CK-II;    casein kinase II;    DTT;    dithiothreitol;    ERp99;    99-kDa endoplasmic reticulum protein;    GST;    glutathione-S-transferase;    HIV-1;    human immunodeficiency virus type 1;    oGA;    glycyrrhetinic acid derivative;    NCS;    neocarzinostatin;    NCS-chrom;    NCS-chromophore;    NLS;    nuclear localization signal;    PMSF;    phenylmethylsulfonyl fluoride;    poly-Arg;    poly-l-arginine;    Rev;    regulator of expression of virion proteins;    rRev;    recombinant Rev;    rRT;    recombinant reverse transcriptase;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;    Tat;    transactivator of transcription;   

DOI  :  10.1016/S0014-5793(98)00538-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The stimulatory effects of several DNA-binding basic proteins (histone and protamine) and HIV-1 Rev with arginine (Arg)-rich clusters on the activity of casein kinase II (CK-II) were investigated in vitro. It was found that recombinant Rev (rRev) and the synthetic oligo-fragments corresponding to the amino acid sequences of its Arg-rich cluster stimulate CK-II activity in a dose-dependent manner. The activated CK-II phosphorylates several cellular and viral proteins in HIV-1 infected human MOLT-4 cells, and also phosphorylates HIV-1 structural proteins, including recombinant reverse transcriptase (rRT). These phosphorylations are selectively inhibited by CK-II inhibitors, such as quercetin, oGA (a glycyrrhetinic acid derivative) and NCS-chrom (an enediyne containing antibiotic). The data presented here suggest that HIV-1 Rev acts as an effective potent activator of CK-II, which may be a cellular mediator promoting HIV-1 replication in virus-infected cells.

【 授权许可】

Unknown