Antigenic determinants synthesized in a library of randomly cloned fragments of the HIV‐1 genome

FEBS Letters

Vasilyev, A.L.¹  Sverdlov, E.D.¹  Nikolayeva, I.A.³  Mikhailov, M.V.²  Arsenyan, S.G.¹ 

[1] Institute of Immunology, USSR Ministry of Public Health, Moscow, USSR;M.M. Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow 117871, USSR;All-Union Scientific Research Institute of Biotechnology, USSR Ministry of Medical Industry, Moscow 117246 USSR

关键词: Human immunodeficiency virus-1;    Immunoepitope;    Expression;    Acquired immunodeficiency syndrome;    Diagnostics;    INF-γ;    human γ-interferon;    IAA;    indole-3-acrylic acid;    a. a.;    amino acid;    pTrp;    tryptophane promoter;   

DOI  :  10.1016/0014-5793(90)80036-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A DNA expression library of randomly selected fragments of the HIV-1 genome was constructed and used to search for antigenic determinants. A large segment of the HIV-1 provirus was sonicated, and 150–250 bp DNA fragments were cloned in a system of expression vectors developed to obtain high yields of recombinant proteins in Escherichia coli. The expressed library was immunoscreened with sera of AIDS patients. Eleven identified immunoreactive clones were found to correspond to already known and new antigenic regions of HIV-1 proteins gp41, p24, and reverse transcriptase.

【 授权许可】

Unknown