Identification and cloning of a novel cellular protein Naf1, Nef‐associated factor 1, that increases cell surface CD4 expression 1

FEBS Letters

Yamamoto, Naoki¹  Tsurutani, Naomi¹  Dixon, Michael J²  Fukushi, Masaya¹  Dixon, Jill²  Kimura, Tohru¹ 

[1] Departments of Microbiology and Molecular Virology, School of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, 113-8519 Tokyo, Japan;School of Biological Sciences, University of Manchester, 3.239, Stopford Building, Oxford Road, Manchester M13 9PT, UK

关键词: Human immunodeficiency virus-1;    Nef;    Naf1;    CD4 expression;   

DOI  :  10.1016/S0014-5793(98)01631-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular protein Naf1 (Nef-associated factor 1), which associated with Nef in the yeast two-hybrid system and pull-down assay. The Naf1 gene generates two isoforms (Naf1α and β) containing four coiled-coil structures. The Naf1 mRNA is ubiquitously expressed in human tissues with strong expression in peripheral blood lymphocytes and spleen. Naf1 overexpression increased cell surface CD4 expression. Nef suppressed this Naf1-induced augmentation of CD4 expression, providing a novel mode of Nef action in CD4 down-regulation.

【 授权许可】

Unknown