Nuclear localization of protein phosphatase 5 is dependent on the carboxy‐terminal region

FEBS Letters

Zeke, Tamás¹  Cohen, Patricia T.W¹  Prescott, Alan R¹  Borthwick, Emma B¹ 

[1] Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, MSI/WTB Complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK

关键词: Protein phosphatase;    Nuclear localization;    Tetratricopeptide repeat;    Tumor promoter;    Cell signalling;   

DOI  :  10.1016/S0014-5793(01)02177-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endogenous and overexpressed protein phosphatase 5 (PP5) localizes to the nucleus and cytoplasm of HeLa cells, while the overexpressed TPR domain of PP5 is restricted to the cytoplasm. Deletion and mutational analysis of human PP5 demonstrates that the C-terminal amino acids 420–499 are essential for nuclear localization and PP5 activity is not required. Since the phosphatase domain terminates at 473, these studies suggest that the highly conserved section (476–491) with the eukaryotic consensus FXAVPHPXΦXPMAYAN is required for nuclear localization of PP5. Bacterially expressed PP5 is inhibited by several tumor promoters but not by the anticancer drug fostriecin.

【 授权许可】

Unknown