期刊论文详细信息
FEBS Letters | |
An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues | |
Klumpp, Susanne1  Cohen, Philip1  Schelling, Donald L.1  | |
[1] Department of Biochemistry, University of Dundee, Dundee, Scotland | |
关键词: Protein phosphatase; Protein phosphorylation; Inhibitor protein; Okadaic acid; Tumor promoter; | |
DOI : 10.1016/0014-5793(89)80803-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020292244ZK.pdf | 415KB | download |