An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues

FEBS Letters

Klumpp, Susanne¹  Cohen, Philip¹  Schelling, Donald L.¹ 

[1] Department of Biochemistry, University of Dundee, Dundee, Scotland

关键词: Protein phosphatase;    Protein phosphorylation;    Inhibitor protein;    Okadaic acid;    Tumor promoter;   

DOI  :  10.1016/0014-5793(89)80803-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.

【 授权许可】

Unknown