Expression and distribution of heme oxygenase‐1 and ‐2 in rat brown adipose tissue: the modulatory role of the noradrenergic system

FEBS Letters

Tonello, Cristina¹  Cancello, Raffaella²  Nisoli, Enzo¹  Carruba, Michele O¹  Giordano, Antonio²  Cinti, Saverio² 

[1] Center for Study and Research on Obesity, Department of Preclinical Sciences, Faculty of Medicine, University of Milan, LITA Vialba, L. Sacco Hospital, Via G.B. Grassi 74, 20157 Milan, Italy;Institute of Normal Human Morphology-Anatomy, Faculty of Medicine, University of Ancona, Via Tronto 10/A, 60020 Ancona, Italy

关键词: Heme oxygenase;    Heat shock protein 32;    Brown adipose tissue;    Noradrenaline;    Rat;   

DOI  :  10.1016/S0014-5793(00)02217-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate whether brown adipose tissue (BAT) expresses the inducible (HO-1) and the constitutive (HO-2) isoform of heme oxygenase, reverse transcriptase-polymerase chain reaction, Western blotting and immunohistochemistry were performed on interscapular BAT (IBAT) from rats acclimated at environmental temperature or exposed to cold. Both HO isoforms were detected in rat IBAT. They were immunolocalized in the cytoplasm and/or nuclei of brown adipocytes, in parenchymal capillaries, arteries and in some veins and nerves. Whereas cold exposure did not affect HO-2 expression, it significantly increased the expression of HO-1, both at mRNA (about 3-fold) and protein (about 2-fold) levels, reflecting the increased expression of HO-1 in the brown adipocytes and endothelial cells of parenchymal capillaries. Western blotting of cytosolic and nuclear protein extracts from cultured differentiated brown adipocytes showed that HO-1 and HO-2 are indeed localized in the cytosol and nuclei of brown adipocytes, and that noradrenaline stimulation significantly increased their amount in cytosol but not in the nuclear fraction.

【 授权许可】

Unknown