Oleuropein aglycone enhances UCP1 expression in brown adipose tissue in high-fat-diet-induced obese rats by activating beta-adrenergic signaling

卷:40

Oi-Kano, Yuriko ; Iwasaki, Yusaku ; Nakamura, Toshiyuki ; Watanabe, Tatsuo ; Goto, Tsuyoshi ; Kawada, Teruo ; Watanabe, Kenichi ; Iwai, Kazuo

2-1 Aoyama

关键词: Brown adipose tissue;    Noradrenaline;    Oleuropein aglycone;    TRPA1;    TRPV1;    Uncoupling protein 1;   

DOI  :  10.1016/j.jnutbio.2016.11.009

学科分类：食品科学和技术

【 摘 要 】

Oleuropein is the pungent principle of raw olives. Oleuropein aglycone (OA) is a major phenolic compound in extra virgin olive oil and the absorbed form of oleuropein. We aimed to determine the mechanism underlying the nutritional effects of oleuropein and OA on interscapular brown adipose tissue (IBAT) in rats with high-fat (HF) diet-induced obesity by examining the agonistic activity of oleuropein and OA toward the transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1). Four week-old male Sprague Dawley rats were fed an HF (palm oil 30% wt:wt) diet alone or with oleuropein (HF-0,1 g/kg diet) for 28 days. In rats fed HF-0 compared to HF, urinary noradrenaline, adrenaline and UCPI levels in1BAT were significantly higher, whereas plasma leptin levels and the total weight of the abdominal cavity adipose tissue were significantly lower. In anaesthetized 7-week-old male Sprague Dawley rats, the OA (3.8 mg of intravenous injection)-induced increase in plasma noradrenaline secretion was suppressed by TRPA1 or TRPV1 antagonist and by beta 2- or beta 3-adrenoceptor antagonist. Furthermore, OA-activated rat and human TRPV1 s expressed on HEK293 cells at the same level as zingerone (pungent component in ginger). OA also activated humanTRPA1, and its potency was approximately 10-fold stronger than that for TRPV1. These findings suggest that OA is the agonist of both TRPA1 and TRPV1 and that OA enhances UCPI expression in IBAT with a concomitant decrease in the visceral fat mass of HF-diet-induced obese rats through enhanced noradrenaline secretion via beta-adrenergic action following TRPAI and TRPV1 activation. (C) 2016 Elsevier Inc. All rights reserved.