Modulation of WHSC2 expression in human endothelial cells

FEBS Letters

Maier, J.A.M¹  Manganini, M¹  Mariotti, M¹ 

[1] Department of Preclinical Sciences-LITA Vialba, University of Milano, Via G.B. Grassi, 74, 20157 Milan, Italy

关键词: WHSC2;    Endothelial cell;    HIV-1 Tat;    Angiogenesis;    WHS;    Wolf–Hirschhorn syndrome;    HLH;    helix-loop-helix;    NLS;    nuclear localization sequence;    NELF;    negative elongation factor;   

DOI  :  10.1016/S0014-5793(00)02335-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

WHSC2, a novel gene recently isolated within the critical region of Wolf–Hirschhorn syndrome, is expressed in endothelial cells. WHSC2 is downregulated by HIV-1 Tat, whereas it is not modulated by angiogenic and pro-differentiative molecules. WHSC2 encodes a basic polypeptide of 528 amino acids. The in vitro translated protein shows a molecular weight of 57 kDa. WHSC2 has two nuclear translocation sequences which actively mediate its transport to the nucleus, as shown in whsc2-GFP-transfected NIH-3T3. We also found a helix-loop-helix (HLH) motif in region 130–185. Since members of the HLH family control differentiation and cell cycle progression, we hypothesize that WHSC2 may function as a transcriptional repressor.

【 授权许可】

Unknown