Genomic Exploration of the Hemiascomycetous Yeasts: 14. Debaryomyces hansenii var. hansenii

FEBS Letters

Casaregola, Serge¹  Bon, Elisabeth¹  Artiguenave, François²  Lépingle, Andrée¹  Nguyen, Huu-Vang¹  Neuvéglise, Cécile¹  Wincker, Patrick²  Gaillardin, Claude¹ 

[1] Collection de Levures d'Intérêt Biotechnologique, Laboratoire de Génétique Moléculaire et Cellulaire, INA-PG, INRA UMR216, CNRS URA1925, F-78850 Thiverval-Grignon, France;Genoscope, Centre National de Séquençage, 2 rue Gaston Crémieux, BP191, F-91057 Evry Cedex, France

关键词: Non-conventional yeast;    Ribosomal DNA;    Mitochondrial DNA;    Retrotransposon;   

DOI  :  10.1016/S0014-5793(00)02285-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By analyzing 2830 random sequence tags (RSTs), totalling 2.7 Mb, we explored the genome of the marine, osmo- and halotolerant yeast, Debaryomyces hansenii. A contig 29 kb in length harbors the entire mitochondrial genome. The genes encoding Cox1, Cox2, Cox3, Cob, Atp6, Atp8, Atp9, several subunits of the NADH dehydrogenase complex 1 and 11 tRNAs were unambiguously identified. An equivalent number of putative transposable elements compared to Saccharomyces cerevisiae were detected, the majority of which are more related to higher eukaryote copia elements. BLASTX comparisons of RSTs with databases revealed at least 1119 putative open reading frames with homology to S. cerevisiae and 49 to other genomes. Specific functions, including transport of metabolites, are clearly over-represented in D. hansenii compared to S. cerevisiae, consistent with the observed difference in physiology of the two species. The sequences have been deposited with EMBL under the accession numbers AL436045–AL438874.

【 授权许可】

Unknown