Widespread effect of N-acetyl-d-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

Microbial Cell Factories

Daisuke Sasaki¹  Tomohisa Hasunuma¹  Akihiko Kondo¹  Kentaro Inokuma¹  Mami Matsuda¹ 

[1] Graduate School of Science, Technology and Innovation, Kobe University;

关键词: Metabolome analysis;    N-acetylglucosamine;    Glucose;    Xylose;    Scheffersomyces stipitis;    Non-conventional yeast;   

DOI  :  10.1186/s12934-018-0998-4

来源: DOAJ

【 摘 要 】

Abstract Background Following cellulose, chitin is the most abundant renewable resource and is composed of the monomeric amino sugar N-acetyl-d-glucosamine (GlcNAc). Although many yeasts, including Saccharomyces cerevisiae, have lost their ability to utilize GlcNAc, some yeasts are able to use GlcNAc as a carbon source. However, our understanding of the effects of GlcNAc on the intracellular metabolism of nitrogen-containing compounds in these yeast species is limited. Results In the present study, we quantitatively investigated the metabolic responses to GlcNAc in the GlcNAc-assimilating yeast Scheffersomyces stipitis (formerly known as Pichia stipitis) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The comprehensive analysis of the metabolites extracted from S. stipitis cells grown in glucose, xylose, or GlcNAc revealed increased intracellular accumulation of a wide range of nitrogen-containing compounds during GlcNAc assimilation in this yeast. The levels of aromatic, branched-chain, and sulfur-containing amino acids and adenine, guanine, and cytosine nucleotides were the highest in GlcNAc-grown cells. Conclusions The CE-TOFMS analysis revealed a positive effect for GlcNAc on the intracellular concentration of a wide range of nitrogen-containing compounds. The metabolomic data gathered in this study will be useful for designing effective genetic engineering strategies to develop novel S. stipitis strains for the production of valuable nitrogen-containing compounds from GlcNAc.

【 授权许可】

Unknown