Genetic diversity of the endangered species Sphyrna lewini (Griffith and Smith 1834) in Lombok based on mitochondrial DNA

1st International Conference on Fisheries and Marine Science

Hadi, S.^1 ; Anggraini, N.P.^1 ; Muttaqin, E.^2 ; Simeon, B.M.^2 ; Subhan, B.^1 ; Madduppa, H.^1

Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java

16680, Indonesia^1

Wildlife Conservation Society, Bogor, West Java, Indonesia^2

关键词: DNA amplification;    Endangered species;    Haplotype diversity;    Mitochondrial DNA;    Molecular approach;    Phylogenetic analysis;    Phylogenetic reconstruction;    Special treatments;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/236/1/012024/pdf
DOI  :  10.1088/1755-1315/236/1/012024

来源: IOP

【 摘 要 】

Sphyrna lewini is a shark with a wide distribution and one of the most endangered species. Although The International Union for Conservation of Nature (IUCN) classified this species as endangered and the species was already protected by law in Indonesia, S. lewini are currently high exploited because of their high economic value. This study conducted to analyze the genetic diversity and phylogenetic reconstruction the S. lewini landed in Lombok (n = 30) using a molecular approach based on the mitochondrial DNA. DNA amplification with the CO1 gene successfully identified 30 individuals of S. lewini with an average 625-bp nucleotide length. The value of haplotype diversity in Lombok (Hd) = 0.40 and nu π = 0.0018. The results of phylogenetic analysis using Neighbor-Joining (NJ) by Kimura 2-parameter model with 1000 bootstrap showed the connectivity of S. lewini landed in Lombok in 1 clade of 5 haplotypes. This study showed that low genetic diversity and significant differences in genetic structure between populations made this species vulnerable to extinction in Indonesia and required special treatment.