Genomic Exploration of the Hemiascomycetous Yeasts: 2. Data generation and processing

FEBS Letters

Duprat, Simone¹  Saurin, William¹  Scarpelli, Claude¹  Jovelin, Fabien¹  Artiguenave, François¹  Weissenbach, Jean¹  Brottier, Phillippe¹  Wincker, Patrick¹  Verdier, Jean¹  Vico, Virginie¹ 

[1] GENOSCOPE, Centre National de Séquençage, 2 rue Gaston Crémieux, P.O. Box 91, F-Evry Cedex, France

关键词: High-throughput sequencing;    Sequence analysis;    Genome;    Hemiascomycete;   

DOI  :  10.1016/S0014-5793(00)02273-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.

【 授权许可】

Unknown