Use of ruthenium red as an inhibitor of mitochondrial Ca2+ uptake in single rat cardiomyocytes

FEBS Letters

Griffiths, Elinor J¹ 

[1] Bristol Heart Institute, Department of Cardiac Surgery, Level 7, Bristol Royal Infirmary, University of Bristol, Bristol BS2 8HW, UK

关键词: Indo-1;    Mitochondrion;    Calcium;    Ruthenium red;    RR;    ruthenium red;    [Ca2+]i;    intracellular [Ca2+];    [Ca2+]m;    mitochondrial [Ca2+];    [Ca2+]c;    cytosolic [Ca2+];   

DOI  :  10.1016/S0014-5793(00)02268-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

With the current resurgence of interest in the role of mitochondrial [Ca²⁺] in energy production and cellular Ca²⁺ signalling, ruthenium red (RR) is being increasingly used as an inhibitor of mitochondrial Ca²⁺ uptake. In the present study, the effects of RR on cell and mitochondrial [Ca²⁺], and on cell contractility were determined in isolated rat ventricular myocytes subjected to adrenergic and electrical stimulation. At low concentrations, 0–1 μM, RR inhibited mitochondrial Ca²⁺ uptake but this was a secondary effect due to a reduced total intracellular [Ca²⁺], a conclusion supported by the ability of RR to inhibit cell shortening. 5 μM RR completely inhibited cell contraction, whereas higher concentrations, 10–25 μM, induced spontaneous Ca²⁺ oscillations and contractile waves. These results indicate that great care must be taken when using RR in intact cells, and in interpreting any effects as resulting from a primary inhibition of mitochondrial Ca²⁺ uptake.

【 授权许可】

Unknown