Effects of the membrane potential upon the Ca2+‐ and cumene hydroperoxide‐induced permeabilization of the inner mitochondrial membrane

FEBS Letters

Kushnareva, Yulia E.¹  Novgorodov, Sergey A.¹  Eriksson, Ove²  Gudz, Tatjana I.³  Leikin, Yury N.⁴ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, School of Biology, Moscow State University, Moscow 119899, U.S.S.R.;Department of Medical Chemistry, University of Helsinki, Helsinki, Finland;Department of Biophysics, School of Biology, Moscow State University, Moscow 119899, U.S.S.R.;Department of Biochemistry, School of Biology, Moscow State University, Moscow 119899, U.S.S.R.

关键词: Mitochondrion;    Non-specific permeability;    Ca2+;    ΔΨ;    mitochondrial inner-membrane potential;    CATR;    carboxyatractiloside;    TPP+;    tetraphenylphosphonium;    RLM;    rat liver mitochondria;    RR;    Ruthenium red;    CuOOH;    cumene hydroperoxide;    FCCP;    carbonylcyanide-4-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(91)81389-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protonophore-induced ΔΨ decrease in a 180-140 mV range causes an increase in the lag-period of Ca²⁺-induced mitochondrial permeabilization but has little effect on the cumene hydroperoxide-induced permeability transition of mitochondria. Suppression of the non-specific permeability induction seems to be mediated by an increase in [ADP] in the mitochondrial matrix. A further decrease in ΔΨ leads to additional suppression of the non-specific permeability as a result of a partial ruthenium red-sensitive efflux of the previously accumulated Ca²⁺. On the other hand, complete dissipation of ΔΨ causes immediate induction of the non-specific permeability. It is concluded that only complete dissipation of ΔΨ caused by H⁺ leakages may act as a trigger for non-specific permeability induction.

【 授权许可】

Unknown