New molecular aspects of regulation of mitochondrial activity by fenofibrate and fasting

FEBS Letters

Wrutniak-Cabello, Chantal³  Rodier, Anne³  Daury, Laetitia³  Cabello, Gérard³  Pineau, Thierry²  Rochard, Pierrick³  Dauça, Michel¹  Casas, François³ 

[1] Laboratoire de Biologie Cellulaire du Développement, EA 2402 ‘Proliférateurs de Peroxysomes’, Université Henri Poincaré-Nancy I, Faculté des Sciences, P.O. Box 239, 54506 Vandoeuvre-lès-Nancy Cedex, France;Laboratoire de Pharmacologie Toxicologie, Institut National de la Recherche Agronomique (INRA), 180 Chemin de Tournefeuille, P.O. Box 3, 31931 Toulouse Cedex 9, France;UMR Différenciation Cellulaire et Croissance (INRA-UMII-ENSAM), Unité d'Endocrinologie Cellulaire, Institut National de la Recherche Agronomique (INRA), place Viala, 34060 Montpellier Cedex 1, France

关键词: Mitochondrion;    Fibrate;    Fasting;    Mitochondrial T3 receptor;    Peroxisome proliferator activated receptor;   

DOI  :  10.1016/S0014-5793(00)02023-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fenofibrate and fasting are known to regulate several genes involved in lipid metabolism in a similar way. In this study measuring several mitochondrial enzyme activities, we demonstrate that, in contrast to citrate synthase and complex II, cytochrome c oxidase (COX) is a specific target of these two treatments. In mouse liver organelles, Western blot experiments indicated that mitochondrial levels of p43, a mitochondrial T3 receptor, and mitochondrial peroxisome proliferator activated receptor (mt-PPAR), previously described as a dimeric partner of p43 in the organelle, are increased by both fenofibrate and fasting. In addition, in PPARα-deficient mice, this influence was abolished for mt-PPAR but not for p43, whereas the increase in COX activity was not altered. These data indicate that: (1) PPARα is involved in specific regulation of mt-PPAR expression by both treatments; (2) fenofibrate and fasting regulate the mitochondrial levels of p43 and thus affect the efficiency of the direct T3 mitochondrial pathway.

【 授权许可】

Unknown