Regulation of p85α phosphatidylinositol‐3‐kinase expression by peroxisome proliferator‐activated receptors (PPARs) in human muscle cells

FEBS Letters

Chevillotte, Emmanuel¹  Rieusset, Jennifer¹  Vidal, Hubert¹  Bouzakri, Karim¹  Roques, Marina¹ 

[1] INSERM U449, Faculté de Médecine René Laennec, Université Claude Bernard Lyon-1, 69372 Lyon, France

关键词: Nuclear receptor;    Thiazolidinedione;    Fibrate;    Insulin resistance;    PPAR;    peroxisome proliferator-activated receptor;    PPRE;    peroxisome proliferator-responsive element;    RT-cPCR;    reverse transcription-competitive polymerase chain reaction;    p85αPI-3K;    p85α-regulatory subunit of phosphatidylinositol-3-kinase;    9-cis-RA;    9-cis-retinoic acid;   

DOI  :  10.1016/S0014-5793(01)02674-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Regulation of p85α phosphatidylinositol-3-kinase (p85αPI-3K) expression by peroxisome proliferator-activated receptor (PPAR) activators was studied in human skeletal muscle cells. Activation of PPARγ or PPARβ did not modify the expression of p85αPI-3K. In contrast, activation of PPARα increased p85αPI-3K mRNA. This effect was potentiated by 9-cis-retinoic acid, an activator of RXR. Up-regulation of p85αPI-3K gene expression resulted in a rise in p85αPI-3K protein level and in an increase in insulin-induced PI3-kinase activity. According to the role of p85αPI-3K in insulin action, these results suggest that drugs with dual action on both PPARγ and PPARα can be of interest for the treatment of insulin resistance.

【 授权许可】

Unknown