Normalization of the levels of inflammatory molecules in Mycobacterium smegmatis -infected U937 cells by fibrate pretreatment

Biological research: BR

Sung-Jo Kim¹  Minho Hong¹  Sungweon Ryoo²  Tae-Hwe Heo³  Ki Duk Song⁴  Hak-Kyo Lee⁴ 

[1] Department of Biotechnology, Hoseo University, Chungnam, Republic of Korea;Korean Institute of Tuberculosis, Gangoe-myeon, Cheongwon-gun,Republic of Korea;Laboratory of Immunology, Integrated Research Institute of Pharmaceutical Sciences, College of Pharmacy, The Catholic University of Korea, Bucheon, Republic of Korea;The Animal Genomics and Breeding Center, Han-Kyong National University, Anseong, Republic of Korea

关键词: Fibrate;    MyD88;    TNF-α;    Tuberculosis;   

DOI  :  10.1186/0717-6287-47-42

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Tuberculosis (TB) is a respiratory tract disease caused by Mycobacterium tuberculosis infection. M. tuberculosis exploits immune privilege to grow and divide in pleural macrophages. Fibrates are associated with the immune response and control lipid metabolism through glycolysis with β-oxidation of fatty acids. In this study, we investigated the effect of fibrate pretreatment on the immune response during M. smegmatis infection in U937 cells, a human leukemic monocyte lymphoma cell line. The protein expression of tumor necrosis factor α (TNF-α), an inflammatory marker, and myeloid differentiation primary response gene 88 (MyD88), a toll like receptor adaptor molecule, in the infected group increased at 1 and 6 h after M. smegmatis infection of U937 cells. Acetyl coenzyme A acetyl transferase-1 (ACAT-1), peroxisome proliferator-activated receptor-α (PPAR-α), TNF-α, and MyD88 decreased in U937 cells treated with fibrates at 12 and 24 h after treatment. More than a 24 h pretreatment with fibrate resulted in similar expression levels of ACAT-1 and PPAR-α between infected vehicle control and infected groups which were pretreated with fibrate for 24 h. However, upon exposure to M. smegmatis, the cellular expression of the TNF-α and MyD88 in the infected groups pretreated with fibrate for 24 h decreased significantly compared to that in the infected vehicle group. These results suggest that fibrate pretreatment normalized the levels of inflammatory molecules in Mycobacterium smegmatis-infected U937 cells. Further studies are needed to confirm the findings on pathophysiology and immune defense mechanism of U937 by fibrates during M. tuberculosis infection.

【 授权许可】

CC BY