In vitro nuclear reconstitution could be induced in a plant cell‐free system

FEBS Letters

Tao, Wei¹  Wu, Min¹  Liu, Xiaoling¹  Zhao, Yun¹  Zhai, Zhonghe¹ 

[1] College of Life Sciences, Peking University, Beijing 100871, PR China

关键词: Carrot cell;    Cell-free system from plant;    Demembranated sperm chromatin;    Nuclear reconstitution;    Nucleosome;    EGTA;    ethylene glycol-bis(β-amino ethyl ether)-N;    N′-tetraacetic acid;    DTT;    dithiothreitol;    PMSF;    phenylmethylsulfonyl fluoride;    HCG;    human chorionic gonadotropin;    XN buffer;    Xenopus sperm buffer;    BSA;    bovine serum albumin;    HEPES;    N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid];    DAPI;    4′6-diamidino-2-phenylindole;   

DOI  :  10.1016/S0014-5793(00)01938-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cell-free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus. Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double-layered nuclear membrane and nuclear pores. Micrococcal nuclease treatment indicates that remodeling of the demembranated sperm chromatin has occurred and the structure of nucleosome is formed during nuclear reconstitution. These data indicate that the nuclear reconstitution can be induced in cell-free systems from plants, and the self-assembly of the nucleus is ubiquitous in both animal and plant cells.

【 授权许可】

Unknown