Apoptosis of mouse liver nuclei induced in the cytosol of carrot cells

FEBS Letters

Zhao, Yun¹  Sun, Ying-Li¹  Jiang, Zheng-Fan¹  Zhai, Zhong-He¹ 

[1]Life Science College, Peking University, Beijing 100871, China

关键词: Apoptosis;    Carrot cell;    Cytosol;    Nucleus;    Lamin;    TUNEL;    terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labelling;    AC-DEVD-CHO;    N-acetyl-Asp-Glu-Val-Asp-Ala;    AC-YVAD-CHO;    N-acetyl-Try-Val-Ala-aspartinal;    MES;    4-morpholinethansulfonsaure;    EDTA;    disodium ethylene diamine tetracetate;    EGTA;    ethylene glycol-bis(β-amino ethyl ether)-N;    N′-tetra acetic acid;    DTT;    dithiothreitol;    PIPES;    piperazine-N;    N′-bis(2-ethanesulfonic acid)/1;    4-piperazine-diethanesulfonic acid;    DAPI;    4′;    6-diamidino-2-phenylindole;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    ICE;    interleukin-converting enzyme;   

DOI  :  10.1016/S0014-5793(99)00352-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report here the apoptosis of mouse liver nuclei induced in the cytosol of carrot cells by cytochrome c. Several typical characteristics of apoptosis, such as chromatin condensation, margination and apoptotic bodies, were detected. The result of DNA gel electrophoresis showed that DNA was degraded into nucleosomal fragments. The terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labelling procedure was also performed to detect the breakage of 3′-OH ends of a DNA strand. Furthermore, we found that nuclear lamins were degraded from 88 kDa and 66 kDa to 37 kDa and 47 kDa fragments. The DNA fragmentation could be inhibited by AC-DEVD-CHO and AC-YVAD-CHO. The results indicate that the apoptosis in plant cells may share some similar pathways to apoptosis in animal cells.

【 授权许可】

Unknown