Ionic transport in the plasma membrane of carrot protoplasts from embryogenic cell‐suspension cultures

FEBS Letters

Gambale, Franco²  LoSchiavo, Fiorella¹  Bregante, Monica² 

[1] Dipartimento di Biologia, Università di Padova, Via Trieste 75, 35121 Padova, Italy;Istituto di Cibernetica e Biofisica, CNR, Via DeMarini 6, 16149 Genova, Italy

关键词: Ionic channel;    Protoplast;    Carrot cell;   

DOI  :  10.1016/0014-5793(96)00016-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ionic transport properties of protoplasts obtained from embryogenic carrot suspension cells were studied by the patch-clamp technique. In the whole-cell configuration, carrot protoplasts presented macroscopic time-dependent outward currents, showing kinetics of activation which did not depend appreciably on the amplitude of the stimulus. Time- and voltage-dependent whole-cell inward rectifying currents as well as instantaneous non-selective currents were also observed. Both time-dependent inward and outward currents are carried by potassium ions. In a cell-attached configuration, two types of single-channel signals, displaying conductances of 10 and 17 pS, were observed; the instantaneous 10 pS channel was also present in outside-out excised patches.

【 授权许可】

Unknown