FEBS Letters | |
Ionic transport in the plasma membrane of carrot protoplasts from embryogenic cell‐suspension cultures | |
Gambale, Franco2  LoSchiavo, Fiorella1  Bregante, Monica2  | |
[1] Dipartimento di Biologia, Università di Padova, Via Trieste 75, 35121 Padova, Italy;Istituto di Cibernetica e Biofisica, CNR, Via DeMarini 6, 16149 Genova, Italy | |
关键词: Ionic channel; Protoplast; Carrot cell; | |
DOI : 10.1016/0014-5793(96)00016-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ionic transport properties of protoplasts obtained from embryogenic carrot suspension cells were studied by the patch-clamp technique. In the whole-cell configuration, carrot protoplasts presented macroscopic time-dependent outward currents, showing kinetics of activation which did not depend appreciably on the amplitude of the stimulus. Time- and voltage-dependent whole-cell inward rectifying currents as well as instantaneous non-selective currents were also observed. Both time-dependent inward and outward currents are carried by potassium ions. In a cell-attached configuration, two types of single-channel signals, displaying conductances of 10 and 17 pS, were observed; the instantaneous 10 pS channel was also present in outside-out excised patches.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020302310ZK.pdf | 461KB | download |