A δ opioid receptor lacking the third cytoplasmic loop is generated by atypical mRNA processing in human malignomas

FEBS Letters

Mayer, Peter¹  Teschemacher, Hansjörg²  Tischmeyer, Helga¹  Höllt, Volker¹  Gollnick, Harald³  Bonnekoh, Bernd³  Jayasinghe, Melany² 

[1] Institute for Pharmacology and Toxicology, Otto von Guericke University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany;Rudolf Buchheim Institute for Pharmacology, University of Giessen, Giessen, Germany;Clinic for Dermatology and Venereology, University of Magdeburg, Magdeburg, Germany

关键词: δ Opioid receptor;    Melanocyte;    Melanoma;    Skin;    Neuroblastoma;    mRNA processing;   

DOI  :  10.1016/S0014-5793(00)01929-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

δ Opioid receptors were identified in human melanomas by RT-PCR and radioligand binding. In all tumors an additional PCR amplificate was detected in which 144 bp within the third exon were deleted. This fragment corresponded to the third cytoplasmic domain of the receptor protein. The short variant resulted from atypical mRNA processing. There were no common splice recognition sequences around the deleted fragment; instead its excision resembled the removal of a transposon. The deletion was not detected in normal human melanocytes nor in human or rat brain. However, it was present in a human neuroblastoma cell line (SH-SY5Y). Thus, it appears that the occurrence of the short δ opioid receptor is correlated to malignancy.

【 授权许可】

Unknown