Differential gene expression in mesothelioma

FEBS Letters

Leikauf, G.D.¹  Rihn, B.H.⁴  McDowell, S.A.¹  Loubinoux, J.²  Mohr, S.⁴  Binet, S.⁴  Galateau, F.⁵  Keith, G.³ 

[1] University of Cincinnati, P.O. Box 670056, Cincinnati, OH 45267-0056, USA;CHU de Brabois, 54501 Vandoeuvre, France;Institut de Biologie Moléculaire et Cellulaire (IBMC) du CNRS, 67084 Strasbourg, France;Institut National de Recherche et de Sécurité (INRS), P.O. Box 27, 54501 Vandoeuvre, France;CHU Côte de Nacre, 14000 Caen, France

关键词: Mesothelioma;    Mesothelial cell;    cDNA microarray;    High-density filter array;    Cancer marker;   

DOI  :  10.1016/S0014-5793(00)01913-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the molecular events controlling malignant transformation of human pleural cells, we compared constitutive gene expression of mesothelioma cells to that of pleural cells. Using cDNA microarray and high-density filter array, we assessed expression levels of >6500 genes. Most of the highly expressed transcripts were common to both cell lines and included genes associated with stress response and DNA repair, outcomes consistent with the radio- and chemo-resistance of mesothelioma. Interestingly, of the fewer than 300 genes that differed between cell lines, most functioned in (i) macromolecule stability, (ii) cell adhesion and recognition, (iii) cell migration (invasiveness), and (iv) extended cell division. Expression levels of several of these genes were confirmed by RT-PCR and could be useful as diagnostic markers of human mesothelioma.

【 授权许可】

Unknown