BMC Gastroenterology | |
Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, in vitro and in vivo | |
Hui-Mian Xu1  Feng Li2  Zhi-Feng Miao1  Zhe Sun1  Cheng-Gang Jiang1  Yan Xu1  Fu-Nan Liu1  Zhi-Dong Lv1  Di Na1  | |
[1] Department of Oncology, The First Affiliated Hospital, China Medical University, Shenyang, 110001, Liaoning Province, China;Department of Cell Biology, China Medical University, Shenyang, 110001, Liaoning Province, China | |
关键词: Fibrosis; Apoptosis; Mesothelial cell; Stomach neoplasms; Peritoneal carcinomatosis; | |
Others : 1113102 DOI : 10.1186/1471-230X-12-34 |
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received in 2011-07-04, accepted in 2012-04-20, 发布年份 2012 | |
【 摘 要 】
Background
In this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells in vitro and in vivo.
Methods
HMrSV5, a human peritoneal mesothelial cell line, was incubated with supernatants from gastric cancer cells. Morphological changes of HMrSV5 cells were observed. Apoptosis of HMrSV5 cells was observed under a transmission electron microscope and quantitatively determined by MTT assay and flow cytometry. Expressions of apoptosis-related proteins (caspase-3, caspase-8, Bax, bcl-2) were immunochemically evaluated.
Results
Conspicuous morphological changes indicating apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. In vivo, peritoneal tissues treated with gastric cancer cell supernatant were substantially thickened and contained extensive fibrosis.
Conclusions
These findings demonstrate that supernatants of gastric cancer cells can induce apoptosis and fibrosis in HMrSV5 human peritoneal mesothelial cells through supernatants in the early peritoneal metastasis, in a time-dependent manner, and indicate that soluble factors in the peritoneal cavity affect the morphology and function of mesothelial cells so that the resulting environment can become favorable to peritoneal metastases.
【 授权许可】
2012 Na et al.; licensee BioMed Central Ltd.
【 预 览 】
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