Production of endothelin‐1 and big‐endothelin‐1 by pleural mesothelial cells

FEBS Letters

Kuwahara, Masayoshi³  Kuwahara, Maki²  Suzuki, Nobuhiro¹ 

[1] Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., 7 Wadai, Tsukuba Ibaraki 300-42, Japan;Division of Toxicology, Institute of Environmental Toxicology, Suzuki-cho 2-772, Kodaira-shi, Tokyo 187 Japan;Department of Comparative Pathophysiology, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan

关键词: Endothelin;    Big-endothelin;    Mesothelial cell;    Pleura;    Rat;   

DOI  :  10.1016/0014-5793(92)80013-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoreactive endothelin-1 (ET-1) and big-endothelin-1 (big-ET-1) were detected in conditioned medium of cultured rat pleural mesothelial cells by using sensitive sandwich-type enzyme immunoassays. The amount of both ET-1 and big-ET-1 increased time-dependently. In both instances, maximal amount was detectable in conditioned medium obtained after 72 h in culture (ET-1: 117.1 ± 30.1 pg/10⁶ cells; big-ET-1: 2.4 ± 2.1 pg/10⁶ cells). Fetal calf serum markedly stimulated the production of both ET-1 and big-ET-1.

【 授权许可】

Unknown