Cloning and characterization of rat casein kinase 1ϵ

FEBS Letters

Okada, Masato¹  Buijs, Ruud M.³  Kani, Shuichi²  Nagai, Katsuya¹  Takano, Atsuko¹  Shimizu, Kimiko¹ 

[1] Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamada-Oka, Suita, Osaka 565-0871, Japan;Department of Biochemistry, Kobe University School of Medicine, Kobe 650-0017, Japan;Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ Amsterdam, The Netherlands

关键词: Circadian rhythm;    Differential display;    Suprachiasmatic nucleus;    Per;    Phosphorylation;    Nuclear translocation;   

DOI  :  10.1016/S0014-5793(00)01755-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1ϵ (CK1ϵ) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1ϵ and characterization of the protein products. The rCK1ϵ is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells.

【 授权许可】

Unknown