Translocation of the interleukin‐1 receptor‐associated kinase‐1 (IRAK‐1) into the nucleus

FEBS Letters

Brigelius-Flohé, R¹  Kreuzer, O.J¹  Böl, G.-F¹ 

[1] German Institute of Human Nutrition, D-14558 Potsdam-Rehbrücke, Germany

关键词: Interleukin-1;    Interleukin-1 receptor type I;    Interleukin-1 receptor-associated kinase-1;    Translocation;    Nucleus;    IL-1;    interleukin-1;    IL-1RI;    IL-1 receptor type I;    IRAK-1;    IL-1 receptor-associated kinase-1;   

DOI  :  10.1016/S0014-5793(00)01759-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin-1 (IL-1) signal transduction involves the recruitment of the IL-1 receptor-associated kinase-1 (IRAK-1). Subsequent signaling finally leads to nuclear translocation of NFκB. We here show that the association and autophosphorylation of IRAK-1 was already detectable 30 s after IL-1 stimulation of ECV 304 cells. Significant levels of IRAK-1 accumulated in the nucleus 30 min after IL-1 stimulation shown by Western blot analysis and confocal laser scanning microscopy. Nuclear transfer of IRAK-1 upon IL-1 stimulation was confirmed in the murine T cell line EL-4. This characterizes nuclear localization of IRAK-1 as a possibly essential event in the IL-1 signaling cascade.

【 授权许可】

Unknown