Regulation of interleukin‐1 receptors on AtT‐20 mouse pituitary tumour cells

FEBS Letters

Nakai, Yoshikatsu²  Imura, Hiroo²  Segawa, Hajime¹  Fukata, Junichi²  Murakami, Norihiko²  Tominaga, Tomoko²  Ebisui, Osamu²  Fukushima, Mitsuo²  Kobayashi, Hiromasa² 

[1] Second Division, Department of Anesthesia, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Second Division, Department of Internal Medicine, Kyoto University Faculty of Medicine, Kyoto 606, Japan

关键词: Interleukin-1;    AtT-20 cell;   

DOI  :  10.1016/0014-5793(92)80031-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study the cellular mechanisms of interleukin-I (IL-1) in the pituitary corticotroph, we studied the properties of IL-1 receptors on a mouse pituitary ACTH-producing cell line, AtT-20. Scatchard plot analysis revealed a single type of receptor with a K _d (dissociation constant) of 93 pM, and 482 binding sites/cell. [¹²⁵I]IL-1α binding competed with IL-1α and IL-1β in an equimolar fashion. A 24 h pre-incubation with either CRH, epinephrine or nor-epinephrine increased the [¹²⁵I]IL-1α binding sites in the AtT-20 cells and conversely, a similar pre-incubation with either dexamethasone or tumour necrosis factor-α (TNFα) decreased them without affecting the affinity of the receptors in either case.

【 授权许可】

Unknown